pe anti mouse cd36  (Miltenyi Biotec)

Journal: Malaria Journal

Article Title: Acetylsalicylic acid and dihydroartemisinin combined therapy on experimental malaria-associated acute lung injury: analysis of lung function and the inflammatory process

doi: 10.1186/s12936-024-05017-7

Figure Lengend Snippet: CD36 expression on monocytes in MA-ALI. Representative plots illustrating the gating strategy employed for the lung cells analysis. Following the gating on live and singlets cells, CD45 + CD11b + cell subpopulations were identified based on Ly6G and Ly6C expression in neutrophils (Ly6G + Ly6C int CD11b + cells) and inflammatory monocytes (Ly6G − Ly6C hi CD11b + cells) ( A ). Histogram plot displaying CD36 expression on inflammatory monocytes (red filled) ( B ). Percentage of neutrophils ( C ) and inflammatory monocytes ( D ) in the lungs of control and Pb NK65 infected mice treated with vehicle (DMSO), DHA or DHA + ASA. CD36 MFI on inflammatory monocytes ( E ). Data are represented as means ± SEM of 4–7 animals in each group

Article Snippet: They were then washed and incubated for 20 min on ice with the following antibodies: anti-Fc-γ III/II (CD16/32) receptor (2.4G2; BD Pharmingen), FITC anti-mouse CD45 (30-F11; BD, Pharmingen), Alexa Fluor 700 anti-mouse CD11b (M1/70; BD, Pharmingen), APC anti-mouse Ly6G (1A8; BD, Pharmingen), APC Cy7 anti-mouse Ly6C (AL-21; BD, Pharmingen), PE- anti-mouse CD36 (HM36; eBioscience).

Techniques: Expressing, Control, Infection

Journal: Cytometry. Part B, Clinical cytometry

Article Title: Addition of formaldehyde releaser imidazolidinyl urea and MOPS buffer to urine samples enables delayed processing for flow cytometric analysis of urinary cells: A simple, two step conservation method of urinary cells for flow cytometry.

doi: 10.1002/cyto.b.22117

Figure Lengend Snippet: FIGURE 1 Storage of untreated urine samples at 4C reduces staining quality and cellular event count. Loss of detectable immune cells by flow cytometry is observed after incubation in urine over time. (a) Representative pseudocolor plots with gates for CD3+ T cells, CD3+CD4+ and CD3+CD8+ T cells and CD14+CD36+ monocytes/macrophages after various incubation times in urine. Prior gates for lymphocytes and doublet exclusion in scatter dimensions are not shown; Live/dead discrimination was not performed. (b) Respective relative event counts of gates in A for multiple urine samples (color coded). Cellular event counts are displayed as events in relation to events measured immediately after voiding (fresh).

Article Snippet: We used the following monoclonal antibodies for staining: CD4-PEVio770 (clone: REA623), CD14-FITC (clone: TüK4, isotype mouse IgG2a), CD36-PE (clone: AC106, isotype mouse IgG2a), Cytokeratin-FITC (clone: CK3-6H5, isotype mouse IgG1), CD10-PE-Vio770 (clone: 97C5, isotype mouse IgG1), EpCAM-PE (clone: HEA-125, isotype mouse IgG1) (all Miltenyi Biotec), CD3-eFlour780 (clone: SK7, mouse IgG1) (eBioscience), CD8-Alexa647 (clone: GN11/134D7, mouse IgG1) (DRFZ).

Techniques: Staining, Flow Cytometry, Incubation

Journal: Cytometry. Part B, Clinical cytometry

Article Title: Addition of formaldehyde releaser imidazolidinyl urea and MOPS buffer to urine samples enables delayed processing for flow cytometric analysis of urinary cells: A simple, two step conservation method of urinary cells for flow cytometry.

doi: 10.1002/cyto.b.22117

Figure Lengend Snippet: FIGURE 4 Correlation of cellular event counts derived from fresh samples with fixed samples after 1, 3, and 6 days. Cellular event counts of CD4+ T helper cells, monocytes/macrophages (CD14+CD36+) and distal tubular epithelial cells (Cytokeratin+EpCAM+) are displayed. Dashed lines indicate ideal correlation. Pearson's correlation coefficient (R), and p-value displayed. Blue dots indicate samples derived from kidney transplant (NTX) patients, yellow dots indicate samples derived from urinary tract infection (UTI), gray dots indicate samples derived from other kidney diseases.

Article Snippet: We used the following monoclonal antibodies for staining: CD4-PEVio770 (clone: REA623), CD14-FITC (clone: TüK4, isotype mouse IgG2a), CD36-PE (clone: AC106, isotype mouse IgG2a), Cytokeratin-FITC (clone: CK3-6H5, isotype mouse IgG1), CD10-PE-Vio770 (clone: 97C5, isotype mouse IgG1), EpCAM-PE (clone: HEA-125, isotype mouse IgG1) (all Miltenyi Biotec), CD3-eFlour780 (clone: SK7, mouse IgG1) (eBioscience), CD8-Alexa647 (clone: GN11/134D7, mouse IgG1) (DRFZ).

Techniques: Derivative Assay, Infection

Journal: eLife

Article Title: Transcriptome network analysis implicates CX3CR1-positive type 3 dendritic cells in non-infectious uveitis

doi: 10.7554/eLife.74913

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-human CD36-PE Clone: CB38 (mouse monoclonal) , BD , CAT# 555455 , (Dilution:) 1:200.

Techniques: Concentration Assay, Cell Isolation, Isolation, Enzyme-linked Immunosorbent Assay, Recombinant, Sequencing, Software, Flow Cytometry, Purification

Journal: bioRxiv

Article Title: Humanized CD36 mouse model supports the preclinical evaluation of therapeutic candidates targeting CD36

doi: 10.1101/2023.01.09.523279

Figure Lengend Snippet: A: Generation of the hCD36 mouse strain. B: Expression of CD36 mRNA in hCD36 mice; C: Expression of CD36 on peritoneal exudative macrophages in hCD36 mice (n=1,7-week-old).

Article Snippet: CD36 expression was examined in bone marrow derived from WT mice and heterozygous hCD36 mice, and hCD36 mice (peritoneal exudative macrophages) using Anti-mouse CD36 Antibody-APC (Biolegend, 102611) and Anti-mouse CD36-PE (eBioscience TM , 17-0362-82).

Techniques: Expressing

Journal: bioRxiv

Article Title: Humanized CD36 mouse model supports the preclinical evaluation of therapeutic candidates targeting CD36

doi: 10.1101/2023.01.09.523279

Figure Lengend Snippet:

Article Snippet: CD36 expression was examined in bone marrow derived from WT mice and heterozygous hCD36 mice, and hCD36 mice (peritoneal exudative macrophages) using Anti-mouse CD36 Antibody-APC (Biolegend, 102611) and Anti-mouse CD36-PE (eBioscience TM , 17-0362-82).

Techniques: Expressing

Journal: bioRxiv

Article Title: Humanized CD36 mouse model supports the preclinical evaluation of therapeutic candidates targeting CD36

doi: 10.1101/2023.01.09.523279

Figure Lengend Snippet: A: Design of in vivo drug efficacy programs; B: Analysis of the in vitro binding of 1G04 to CD36. C: Tumor volume measurement during the treatment. D: Body weight record during the treatment.

Article Snippet: CD36 expression was examined in bone marrow derived from WT mice and heterozygous hCD36 mice, and hCD36 mice (peritoneal exudative macrophages) using Anti-mouse CD36 Antibody-APC (Biolegend, 102611) and Anti-mouse CD36-PE (eBioscience TM , 17-0362-82).

Techniques: In Vivo, In Vitro, Binding Assay